The biological vacuum

Theory: Everyone knows the gas-free vacuum pack. It guarantees that food will keep for a long time because no life can survive inside a gas-free vacuum. It is easy to create a gas-free vacuum pack. One only needs a vacuum cleaner. If water is free of gases because it is below atmospheric pressure, hardly any living thing can develop in it. All gases escape and do not allow the existence of life.

These facts shed a completely new light onto the basics of biology if one considers them in connection with the following experiments I carried out 15 years ago or the experiments with the free water membrane. And no one wants to print them. It is also a bit my own fault since I considered the experiments only significant in relation to improved capillary conduction within biological media, which is very important but not sufficient. The second relevant fact is that liquids without gas conduct sound by far better. Sparkling wine and mineral water hardly make any noise at all when one pours them into glasses and tossing one glass against the other. In biology, sound is primarily important for the dissemination of information. Furthermore strong ultra-sound is one of the best methods to make water gas-free. Also the phenomenon of sonoluminescence, which leads to a glowing at the border between blue and ultraviolet in the water, is very important in biology and is better carried out in degassed water. Sonoluminescence is the foundation of mitogenetic radiation of GURWITSCH. The main reason, however, is the rejection of all other life forms. A vacuum rejects all kinds of life. Thus immunity defence seems only to be the last means when the vacuum cannot be maintained due to overagitation and its resultant weakness. During the agitation, water-gas (HOH), carbon dioxide (plus numerous other gases but in far lower quantities) are released. Examples are: ethylene, a maturation hormone, or ammonia, which, however, will be bound quickly; in this respect different types of techniques are used. But the most universal method is degassing - the creation of a vacuum pack.

However, also contradictions have to be pointed out. Without gaseous oxygen, which surrounds the organism, there is hardly any successful life. This, however, is not quite correct. Within the cell there is no free oxygen. It is used as hydrogen superoxide. The catalase destroys this hydrogen superoxide immediately but the energy of the oxygen release is used by the peroxidases. Furthermore only the mitochondria respire. They ate only a small fraction of the cell. They have their own DNA-code for their proteins and are not admitted for sexual reproduction. They can best be compared with medieval monasteries, which at that time were the upholders of technological progress with their horticulture, workshops and schools. The monks and nuns lived in strict celibacy. Thus the cell itself does not respire. Also the peroxidases are bound to certain organelles. Within the cell there are numerous specialised factories or microbodies. Also the oxygen of the lungs enters the red blood corpuscles, which cannot divide themselves any further, which is the main function of life. Subsequently they are, in fact, dead. The primary bacteria are furthermore all anaerobic. The fermentation is by far more important and natural than the respiration. The carbon dioxide exists in the basic cell in Carbonate form. The acidification during agitation, however, releases gaseous carbon dioxide. Luckily almost only the outer parts of the plasma are affected. When this happens through radioactive or electromagnetic radiation inside the cell, it has a lethal effect. The inside of a cell is just like our internal body practically free of sense organs, which assume the organisation of the defence. The seeds of life (germs or babies of the cell) can only divide themselves and grow in absolute isolation before they are sent to the exoplasmatic atmospheric front.

The best isolation is the V A C U U M pack

Where is the best vacuum apart from the internal seed kindergarten of the cell? In space. There alive water is flying around everywhere in comets. Comets are almost unpredictable. I think that they assemble in the gravity free zones in the border areas between planets. Also in the gravity free zones between the earth, the moon and the sun a lot of dust and other things assemble. A Polish astronomer has discovered these dirty comers and possible breeding ground for water life.

It goes without saying that there is no absolute vacuum because it would simply be too difficult and uneconomical to produce it. Again there exists an OPTIMUM. The gas concentration in the Ectoplasma is of course by far higher than in the inside and water-gases and others must constantly be driven away by force, this happens mainly due to the buoyancy of the very light water-gases (gaseous HOH as opposed to dense dipole H+ ... OH-).

Here are the old experiments without any modifications. The newly recognised basic idea changes the focus and displays the actual meaning. Introduction:

The publication by ZELEPUCHIN et al. (1) shows that the gas concentration plays a much greater role than the osmotic value of the water. The velocity of the water uptake of detached birch leaves increased sixteen times (!), when the tap water was free of gas. There is a very good correlation between gas concentration and velocity of water uptake.

This effect has nothing to do with the gas free water in the xylem. Also Nitella algae show a strong effect by treatment with degassed water. The germination velocity of seeds increases after treatment with degassed tap water. Degassed distilled water has a much weaker increase of water uptake into leaves than degassed tap water with ions.

The ions play a great role because they give the water structure a higher dense order.

It is not important which physical method of degassing was employed.

We were confronted with this problem as we tested the so called magnetised water. Our tests had shown, that the gas concentration after turbulent running through the magnet transforms itself. The same effect occurred when the water was stirred. The effect has a general relevance in biology. An enhancement of biological activity can be seen after treatment of plant and animal material, in this case cucumber plants and bull sperm.

Material and methods:

The tests were applied to detached leaves of birch and cucumber. We also tested detached top shoots of cucumber and young plants of the same species.

The birch leaves were cut, weighed and then placed with the petioles and lower part in tap and distilled water of different gas concentration. After one or fifteen hours the surface of the leaves was quickly dried between sheets of blotting paper and then weighed.

In table one you can see a few physico-chemical parameters of aerated water.

Table 1: Physico-chemical parameters of tap water after aerating


  pH-value  conductivity   O2-concentration 
before  7.3  1.17 mS   3.7 cm3/l 
after  8.3  1.18 mS   8.3 cm3/l 

The C02-concentration decreases as indirectly shows the pH-value and direct measurements in relative units.


Results

In table two the increase of fresh matter after treatment with water of different gas and ion concentration is shown.

Table 2: Percentage fresh matter increase of birch leaves after short and long time treatment in water


Normal tap water  1.5%  5.0% 
normal distilled water  1.5%  5.0% 
degassed tap water   22.2% (!)   25.4%  
degassed distilled water   5.6%   -- 
tap water aerated before treatment  1.5%   10.1% 
tap water continually aerated during treatment   1.5%  5,0% 
tap water 5h aerated with oxygen before treatment   1.5%   20,0 % (!) 
ditto but with nitrogen   1,5%  5,0% 
ditto but with carbon dioxide   1.5%  5,0% 

Fresh cut or light wilted cucumber leaves were also placed in degassed or normal tap water. In normal tap water the leaves begin to wilt at once. In degassed water we left the leaves for 24 hours. There was no symptom of wilting. Light wilted leaves increased their Turgor in degassed water but continued to wilt in normal tap water. Several fresh cut leaves after a while in degassed water showed that the edges were glassy.

We placed the cut top shoots of young cucumber plants in degassed water for 4 weeks. After three weeks they all rooted. The Turgor folly maintained but the growth ended. Only the leaves were slightly more unfolded.

We placed young cucumber plants with 4...6 leaves in strong hypertonic solutions of 10 mS conductivity. The damage in the degassed solution was faster and more severe than in the hypertonic normal tap water solution (control).


Discussion

The effect of the degassed water is a specific life phenomenon. Dead leaves take on water but show no effect when the water is degassed. Also the Osmosis velocity through a semipermeable membrane is not dependent on gas concentration.

This very strong effect is very difficult to interpret in the light of the recent theory of water uptake. One hint is, that the protoplasm is relatively gas-free. It costs time to degas the water.

The tap water enriched with oxygen before the treatment of the leaves shows the effect only after fifteen hours. This fact shows that it costs time to degas the water by means of respiration. It is an indirect effect of respiration, then continually aerated water shows no effect., because the gas concentration is constant. It is a pure biophysical effect because the degassed water has a higher order. In the protoplasm the water has also a higher order.

The weak effect of distilled water shows that the ions play a great role. Ions order the water too and have a strong effect on biological activity.

The experiments with cucumber show that the water is penetrating the cell wall. The Turgor is enhanced after treatment with degassed water. Together with the water a more intense ionic uptake takes place as show the experiments with the hypertonic solutions.

It seems to be the first time that a shoot of cucumber has rooted. Detached parts of cucumber plants wilt in normal water.

The general biological relevance of the physical effect of gases in biological organisms shows also the similar effect of many sorts of gases of very strong difference in chemical structure on narcosis.

Literature:

V.D.Zelepuchin; LD.Zelepuchin Kljutsch k "shivoj" vode Alma Ata 1980


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